Mr Dhanasekaran Sakthivel

PhD Student
Department of Biochemistry and Molecular Biology
Monash University

dsak8@student.monash.edu

Research Activities

I successfully completed my PhD from Monash University, Australia in September 2017 under the supervision of A/Prof. David Piedrafita and A/Prof. Travis Beddoe. My research interests are largely directed towards understanding the basis of infection and  immunity. I am looking for an early career opportunity to ameliorate and upgrade my knowledge in the field of infectious diseases.

Techniques/Expertise

  • Demonstrated experience in structural and functional characterisation of proteins involved in complex host-pathogen interplay.
  •  Demonstrated experience in defining the innate immune response to multiple pathogens in
  • animal models.Extensive experience in recombinant proteins production (over 40 gene constructs), biochemical, biophysical characterisation and functional studies.
  • Molecular biology:  Gene cloning and gene expression analysis (mRNA and Protein) and gene expression profiling (qPCR).
  • Protein biochemistry: Affinity purification (IMAC and Pull down), Pull down affinity purification (Protein-protein and Protein-glycan), size exclusion and Ion exchange chromatography (AKTA). Circular Dichroism (CD), Analytical Ultracentrifugation and Glycobiology techniques
  • Protein crystallisation: Setting up crystal screening, cryopreservation and diffraction of protein crystals and structure determination)

Collaborations

Prof Jamie Rossjohn
Dr Matthew Perugini
A/Prof David Piedrafita
Dr Travis Beddoe
Dr Gowtam Chowdary Chalasani

Disease Models

  • BALB/c mice for the propagation of Plasmodium berghei, Plasmodium yoleii, Plasmodium falciparum, plasmodium chabaudi
  • Chicken for the New castle disease virus vaccine
  • Sheep and goat for the propagation of  H. contortus immune response 
  • Bovine for the vaccine studies of Theileria annulata 

Genetically Modified Organisms

E. coli

Other members with similar research interests

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Dr Ben Cocks

Department of Environment and Primary Industries Victorian State Government SEE FULL PROFILE >

Dr Louis Schofield

Infection and Immunity Division Walter and Eliza Hall Institute of Medical Research SEE FULL PROFILE >

A/Prof Michelle Dunstone

Department of Biochemistry and Molecular Biology Monash University SEE FULL PROFILE >

Upcoming Events

  • Oct 7

    VIIN Young Investigator Scientific Presentation Skills Workshop

    Online REGISTER | MORE INFO
  • Oct 9

    DC2022: 16th International Symposium on Dendritic Cells 2022

    Pullman Cairns International REGISTER | MORE INFO
  • Nov 16

    VIIN Young Investigator Symposium 2022

    Monash Institute of Pharmaceutical Sciences MORE INFO