Dr Sonia Ghilas

Research Officer
Department of Microbiology and Immunology
Peter Doherty Institute

ghilas.s@laposte.net

Techniques/Expertise

Animal experience: Mouse handling, injection, harvesting...

Cellular techniques: In vitro differentiation of macrophages and dendritic cells from bone marrow cells, Colony assay, Lentiviral particles production, Cellular transduction and transfection, In vitro activation of T Lymphocytes, In vivo CTL assay, ELISA, ELISpot, Extra and Intra-cellular staining, Flow cytometry (BD Canto, BD LSRII, BD Fortessa and Cytek Aurora)

Imaging: Preparation of frozen organ sections (Cryostat) and fresh organ sections (Vibratome), Labelling and acquisition on a confocal microscope (Zeiss LSM), Organs clearing and imaging by 2-photon microscopy (Olympus).

Molecular/Biochemistry: Cloning, Western blot, DNA and RNA extraction, PCR, qPCR, Plaque assay, Colony assay.

Informatics: Microsoft office, GraphPad Prism, DIVA, Flow Jo, LSM Image Browser, Amaris, EndNote, Adobe illustrator, Genotrack

Disease Models

Mouse model
Viral model: Murine cytomegalovirus
Bacterial model: Listeria monocytogenes
Parasitic model: Plasmodium berghei ANKA. Blood and liver stage.

Upcoming Events

  • Nov 29

    49th ASI Annual Scientific Meeting

    Grand Millenium MORE INFO
  • Dec 1

    World AIDS day

  • Nov 28

    50th ASI Annual Scientific Meeting

    MORE INFO