Dr Sonia Ghilas
Department of Microbiology and Immunology
Peter Doherty Institute
Techniques/ExpertiseAnimal experience: Mouse handling, injection, harvesting...
Cellular techniques: In vitro differentiation of macrophages and dendritic cells from bone marrow cells, Colony assay, Lentiviral particles production, Cellular transduction and transfection, In vitro activation of T Lymphocytes, In vivo CTL assay, ELISA, ELISpot, Extra and Intra-cellular staining, Flow cytometry (BD Canto, BD LSRII, BD Fortessa and Cytek Aurora)
Imaging: Preparation of frozen organ sections (Cryostat) and fresh organ sections (Vibratome), Labelling and acquisition on a confocal microscope (Zeiss LSM), Organs clearing and imaging by 2-photon microscopy (Olympus).
Molecular/Biochemistry: Cloning, Western blot, DNA and RNA extraction, PCR, qPCR, Plaque assay, Colony assay.
Informatics: Microsoft office, GraphPad Prism, DIVA, Flow Jo, LSM Image Browser, Amaris, EndNote, Adobe illustrator, Genotrack
Disease ModelsMouse model
Viral model: Murine cytomegalovirus
Bacterial model: Listeria monocytogenes
Parasitic model: Plasmodium berghei ANKA. Blood and liver stage.